Journal: Biotechnology Progress
Article Title: Optimization of multi‐column chromatography for capture and polishing at high protein load
doi: 10.1002/btpr.70047
Figure Lengend Snippet: Optimization plots of the three different Protein A resins studied for the capture step of mAb. (a), (b), and (c) are the Pareto fronts for MSS, MSPrismA, and MSSpcc, respectively. The optimization was done for batch mode (at c mAb of 5 g/L) and continuous mode (at c mAb of 2.5, 5, 7.5, and 10 g/L). Numbers 1, 3, 4, and 5 represent the results for continuous mode at c mAb of 2.5, 5, 7.5, and 10 g/L, respectively; number 2 represents the results for batch mode at c mAb of 5 g/L. (d) Comparison of the optimizations for continuous chromatography of the three different resins at a 5 g/L concentration of mAb. Concentrations of 7.5 and 10 g/L represent an extrapolation of the feed concentrations for model calibration.
Article Snippet: 1 mL HiTrap® columns of ProA resins Mab Select SuRe (MSS), Mab Select PrismA (MSPrismA), and Mab Select SuRe pcc (MSSpcc) and CEX resins CaptoTM S ImpAct (CaptoS Imp) and SP Sepharose Fast Flow (SP Seph FF) were purchased from Cytiva, Uppsala, Sweden.
Techniques: Comparison, Chromatography, Concentration Assay